| MWexperimental | 64848 | kDa |
| MWexpected | 32 | kDa |
| VPorod | 105 | nm3 |
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log I(s)
1.96×10-1
1.96×10-2
1.96×10-3
1.96×10-4
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s, nm-1
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Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B, The Journal of Physical Chemistry B (2024) DOISASDV62 – Human Haemoglobin (Sigma-Aldrich) in 100mM Sodium Phosphate buffer, pH=7.0, SEC-SAXS
Hemoglobin subunit alphaHemoglobin subunit beta
Protoporphyrin IX containing fe
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Fits and models
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SAXS
data from solutions of
Human Haemoglobin (Sigma-Aldrich) in 100mM Sodium Phosphate buffer, pH=7.0, SEC-SAXS
in
100 mM Sodium Phosphate, pH 7
were collected
using a Eiger R 1M detector
at a sample-detector distance of 0.8 m and
at a wavelength of λ = 1.34 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 500.00 μl sample
at 18 mg/ml was injected at a 0.05 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 20°C.
106 successive
15 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Experimental molecular weight: Size&Shape value from PRIMUS was used. |
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