| MWexperimental | 19 | kDa |
| MWexpected | 15 | kDa |
| VPorod | 48 | nm3 |
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log I(s)
2.80×10-2
2.80×10-3
2.80×10-4
2.80×10-5
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s, nm-1
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Unraveling the molecular grammar and the structural transitions underlying the fibrillation of a viral fibrillogenic domain.
Gondelaud F, Leval J, Arora L, Walimbe A, Bignon C, Ptchelkine D, Brocca S, Mukhopadyay S, Longhi S, Protein Sci 34(3):e70068 (2025) Europe PMCSASDVD8 – Hendra virus protein P/V/W PNT3 low-K domain
Protein W (artificial PNT3 variant - low-kappa)|
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of Hendra virus protein P/V/W PNT3 low-K domain in 20 mM HEPES, 150 mM NaCl, pH 7.2 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 5 mg/ml was injected at a 0.30 ml/min flow rate onto a Agilent AdvanceBio SEC 2.7 µm - 130 Å column at 20°C. 600 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
45 µl were injected on a BioSec 3-300 at a flow rate of 0.3 ml/min in HBS |
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