| MWexperimental | 93 | kDa |
| MWexpected | 32 | kDa |
| VPorod | 131 | nm3 |
|
log I(s)
8.23×10-2
8.23×10-3
8.23×10-4
8.23×10-5
|
s, nm-1
|
Hemoglobin–PEG Interactions Probed by Small-Angle X-ray Scattering: Insights for Crystallization and Diagnostics Applications
Baranova I, Angelova A, Stransky J, Andreasson J, Angelov B, The Journal of Physical Chemistry B (2024) DOISASDVE2 – Human Haemoglobin (Sigma-Aldrich) with 10% PEG2000 in 100mM Sodium Phosphate buffer, pH=7.0, c=5mg/ml
Hemoglobin subunit alphaHemoglobin subunit beta
Protoporphyrin IX containing fe
|
|
|
Fits and models
|
|
|
SAXS
data from solutions of
Human Haemoglobin (Sigma-Aldrich) with 10% PEG2000 in 100mM Sodium Phosphate buffer, pH=7.0, c=5mg/ml
in
100mM Sodium Phosphate buffer with 10% (w/v) PEG2000, pH 7
were collected
using a Eiger R 1M detector
at a sample-detector distance of 0.8 m and
at a wavelength of λ = 1.34 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 5.00 mg/ml was measured
at 20°C.
30 successive
30 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||