| MWexperimental | 23 | kDa |
| MWexpected | 15 | kDa |
| VPorod | 54 | nm3 |
|
log I(s)
1.10×100
1.10×10-1
1.10×10-2
1.10×10-3
|
s, nm-1
|
Unraveling the molecular grammar and the structural transitions underlying the fibrillation of a viral fibrillogenic domain.
Gondelaud F, Leval J, Arora L, Walimbe A, Bignon C, Ptchelkine D, Brocca S, Mukhopadyay S, Longhi S, Protein Sci 34(3):e70068 (2025) Europe PMCSASDVE8 – Hendra virus protein P/V/W PNT3 high-K domain
Protein W (artificial PNT3 variant - high-kappa)|
|
|
Fits and models
|
Rg, nm
|
|
Synchrotron SAXS data from solutions of Hendra virus protein P/V/W PNT3 high-K domain in 20 mM HEPES, 150 mM NaCl, pH 7.2 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.133 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 5 mg/ml was injected at a 0.30 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 20°C. 600 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
50 µl were injected on a BioSec 3-300 at 0.3 ml/min in HBS |
|
|||||||||||||||||||||