S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.

Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV, J Biol Chem 300(9):107627 (2024) Europe PMC

SASDVG2 – Staphylococcal extracellular adherence protein (Eap) bound to human cathepsin-G

Protein map
Cathepsin G
MWexperimental 117 kDa
MWexpected 152 kDa
VPorod 166 nm3
log I(s) 1.45×102 1.45×101 1.45×100 1.45×10-1
Protein map Cathepsin G small angle scattering data  s, nm-1
ln I(s)
Protein map Cathepsin G Guinier plot ln 1.46×102 Rg: 5.0 nm 0 (5.0 nm)-2 s2
(sRg)2I(s)/I(0)
Protein map Cathepsin G Kratky plot 1.104 0 √3 sRg
p(r)
Protein map Cathepsin G pair distance distribution function Rg: 5.5 nm 0 Dmax: 20.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Protein map Cathepsin G MULTIFOXS model
log I(s)
 s, nm-1
Protein map Cathepsin G MULTIFOXS model
Protein map Cathepsin G MULTIFOXS model
log I(s)
 s, nm-1
Protein map Cathepsin G MULTIFOXS model
Protein map Cathepsin G MULTIFOXS model
Protein map Cathepsin G MULTIFOXS model
Synchrotron SAXS data from solutions of Staphylococcal extracellular adherence protein (Eap) bound to human cathepsin-G in 20 mM HEPES, 140 mM NaCl, pH 7.4 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 5 mg/ml was injected at a 0.65 ml/min flow rate onto a Shodex KW-803 column at 4°C. 660 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Protein map (Eap)
Mol. type   Protein
Organism   Staphylococcus aureus (strain Mu50 / ATCC 700699)
Olig. state   Monomer
Mon. MW   50.3 kDa
 
UniProt   Q99QS1 (32-478)
Sequence   FASTA
 
Cathepsin G (CG)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   25.4 kDa
 
UniProt   P08311 (22-245)
Sequence   FASTA