S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.

Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV, J Biol Chem 300(9):107627 (2024) Europe PMC

SASDVH2 – Staphylococcal extracellular adherence protein (Eap) bound to human neutrophil elastase

Protein map
Neutrophil elastase
MWexperimental 103 kDa
MWexpected 144 kDa
VPorod 152 nm3
log I(s) 1.51×101 1.51×100 1.51×10-1 1.51×10-2
Protein map Neutrophil elastase small angle scattering data  s, nm-1
ln I(s)
Protein map Neutrophil elastase Guinier plot ln 1.52×101 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
Protein map Neutrophil elastase Kratky plot 1.104 0 √3 sRg
p(r)
Protein map Neutrophil elastase pair distance distribution function Rg: 3.9 nm 0 Dmax: 10.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Protein map Neutrophil elastase MULTIFOXS model
log I(s)
 s, nm-1
Protein map Neutrophil elastase MULTIFOXS model
Protein map Neutrophil elastase MULTIFOXS model
log I(s)
 s, nm-1
Protein map Neutrophil elastase MULTIFOXS model
Protein map Neutrophil elastase MULTIFOXS model
Protein map Neutrophil elastase MULTIFOXS model
Synchrotron SAXS data from solutions of Staphylococcal extracellular adherence protein (Eap) bound to human neutrophil elastase in 20 mM HEPES, 140 mM NaCl, pH 7.4 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 5 mg/ml was injected at a 0.65 ml/min flow rate onto a Shodex LW-803 column at 20°C. 660 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Protein map (Eap)
Mol. type   Protein
Organism   Staphylococcus aureus (strain Mu50 / ATCC 700699)
Olig. state   Monomer
Mon. MW   50.3 kDa
 
UniProt   Q99QS1 (32-478)
Sequence   FASTA
 
Neutrophil elastase (NE)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   23.3 kDa
 
UniProt   P08246 (31-249)
Sequence   FASTA