Dimerization of ADAR1 modulates site-specificity of RNA editing

Mboukou A, Rajendra V, Messmer S, Mandl T, Catala M, Tisné C, Jantsch M, Barraud P, Nature Communications 15(1) (2024) DOI

SASDVH7 – Third double-stranded RNA-binding domain of Human Double-stranded RNA-specific adenosine deaminase (ADAR1) (residues 716-797, i.e. dsRBD3-short)

Third double-stranded RNA-binding domain of human ADAR1 (residues 716-797, i.e. dsRBD3-short)
MWexperimental 21 kDa
MWexpected 18 kDa
VPorod 27 nm3
log I(s) 1.67×10-2 1.67×10-3 1.67×10-4 1.67×10-5
Third double-stranded RNA-binding domain of human ADAR1 (residues 716-797, i.e. dsRBD3-short) small angle scattering data  s, nm-1
ln I(s)
Third double-stranded RNA-binding domain of human ADAR1 (residues 716-797, i.e. dsRBD3-short) Guinier plot ln 1.67×10-2 Rg: 1.9 nm 0 (1.9 nm)-2 s2
(sRg)2I(s)/I(0)
Third double-stranded RNA-binding domain of human ADAR1 (residues 716-797, i.e. dsRBD3-short) Kratky plot 1.104 0 √3 sRg
p(r)
Third double-stranded RNA-binding domain of human ADAR1 (residues 716-797, i.e. dsRBD3-short) pair distance distribution function Rg: 1.9 nm 0 Dmax: 5.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Third double-stranded RNA-binding domain of human ADAR1 (residues 716-797, i.e. dsRBD3-short) PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of Third double-stranded RNA-binding domain of Human Double-stranded RNA-specific adenosine deaminase (ADAR1) (residues 716-797, i.e. dsRBD3-short) in 20 mM Na-HEPES, 55 mM KOAc, 10 mM NaCl, 1 mM TCEP, pH 7.3 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 10°C. Seven successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Third double-stranded RNA-binding domain of human ADAR1 (residues 716-797, i.e. dsRBD3-short) (dsRBD3-short)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   9.2 kDa
 
UniProt   P55265 (716-797)
Sequence   FASTA
 
PDB ID   7ZJ1