| MWexperimental | 201 | kDa |
| MWexpected | 154 | kDa |
| VPorod | 275 | nm3 |
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log I(s)
1.68×10-1
1.68×10-2
1.68×10-3
1.68×10-4
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s, nm-1
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Histone modification-driven structural remodeling unleashes DNMT3B in DNA methylation.
Cho CC, Huang HH, Jiang BC, Yang WZ, Chen YN, Yuan HS, Sci Adv 11(13):eadu8116 (2025) Europe PMCSASDVJ9 – Human DNA methyltransferase 3 beta DNMT3B (215-853) and DNA methyltransferase 3-like DNMT3L (178-379) in complex with histone H3
Histone H3.3DNA methyltransferase 3 beta (215-853)
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Fits and models
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Synchrotron SAXS
data from solutions of
Human DNA methyltransferase 3 beta DNMT3B (215-853) and DNA methyltransferase 3-like DNMT3L (178-379) in complex with histone H3
in
20 mM Tris, 300 mM NaCl, 1 mM TCEP, 5% glycerol, pH 8
were collected
on the
13A beam line
at the Taiwan Photon Source, NSRRC storage ring
(Hsinchu, Taiwan)
using a Eiger X 1M and Eiger X 9M detector
at a sample-detector distance of 2.5 m and
at a wavelength of λ = 0.0827 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 5.00 mg/ml was measured
at 10°C.
199 successive
0.200 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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