| MWexperimental | 210 | kDa |
| MWexpected | 208 | kDa |
| VPorod | 277 | nm3 |
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log I(s)
2.26×10-1
2.26×10-2
2.26×10-3
2.26×10-4
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s, nm-1
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Computational study of DPP8 and DPP9: fundamental insights and inhibitor design
Olivier Beyens, Yann Sterckx, University of Antwerp PhD thesis c:irua:209673 (2024) URLSASDVK6 – Dipeptidyl peptidase 8 (DPP8) with compound 42
Isoform 1 of Dipeptidyl peptidase 8|
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Fits and models
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Synchrotron SAXS data from solutions of DPP8 with compound 42 in 25 mM HEPES, 150 mM NaCl, 2 mM DTT, pH 7.5 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 40.00 μl sample at 11.9 mg/ml was injected at a 0.20 ml/min flow rate onto a Shodex KW404-4F column at 20°C. 870 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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