| MWexperimental | 21 | kDa |
| MWexpected | 21 | kDa |
| VPorod | 19 | nm3 |
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log I(s)
3.40×10-2
3.40×10-3
3.40×10-4
3.40×10-5
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s, nm-1
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DNMT3B
CHAO-CHENG CHO.SASDVK9 – Pro-Trp-Trp-Pro (PWWP) domain from human DNA (cytosine-5)-methyltransferase 3B (DNMT3B) in complex with histone H3
Histone H3.3DNA (cytosine-5)-methyltransferase 3B Pro-Trp-Trp-Pro (PWWP) domain
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Fits and models
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Synchrotron SAXS
data from solutions of
Pro-Trp-Trp-Pro (PWWP) domain from human DNA (cytosine-5)-methyltransferase 3B (DNMT3B) in complex with histone H3
in
20 mM Tris, 300 mM NaCl, 1 mM TCEP, 5% glycerol, pH 8
were collected
on the
13A beam line
at the Taiwan Photon Source, NSRRC storage ring
(Hsinchu, Taiwan)
using a Eiger X 1M and Eiger X 9M detector
at a sample-detector distance of 2.5 m and
at a wavelength of λ = 0.0827 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 10.00 mg/ml was measured
at 10°C.
107 successive
0.200 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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