A conformational fingerprint for amyloidogenic light chains

Paissoni C, Puri S, Broggini L, Sriramoju M, Maritan M, Russo R, Speranzini V, Ballabio F, Nuvolone M, Merlini G, Palladini G, Hsu S, Ricagno S, Camilloni C, (2025) DOI

SASDVM4 – Immunoglobulin light chain H7

Immunoglobulin light chain H7

MW^experimental	45	kDa
MW^expected	45	kDa
V^Porod	54	nm³

log I(s) 8.11×10² 8.11×10¹ 8.11×10⁰ 8.11×10^-1

Immunoglobulin light chain H7 small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Immunoglobulin light chain H7 Guinier plot

ln 8.11×10² R_g: 2.5 nm 0 (2.5 nm)^-2 s²

(sR_g)²I(s)/I(0)

Immunoglobulin light chain H7 Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 2.5 nm 0 D_max: 8.4 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.3

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.124
0.999

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Immunoglobulin light chain H7 MODELLER model

Synchrotron SAXS data from solutions of immunoglobulin light chain H7 in 20 mM TrisHCL, 150 mM NaCl, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.124423 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.40 mg/ml was measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Immunoglobulin light chain H7 (H7)
Mol. type		Protein
Organism		Homo sapiens
Olig. state		Dimer
Mon. MW		22.3 kDa
Sequence		FASTA

PDB ID		5MUH