| MWexperimental | 199 | kDa |
| MWexpected | 198 | kDa |
| VPorod | 274 | nm3 |
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log I(s)
2.73×102
2.73×101
2.73×100
2.73×10-1
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s, nm-1
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Computational study of DPP8 and DPP9: fundamental insights and inhibitor design
Olivier Beyens, Yann Sterckx, University of Antwerp PhD thesis c:irua:209673 (2024) URLSASDVM6 – Dipeptidyl peptidase 9 (DPP9) with compound 42
Isoform 1 of Dipeptidyl peptidase 9|
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Fits and models
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Synchrotron SAXS data from solutions of DPP9 with compound 42 in 25 mM HEPES, 150 mM NaCl, 2 mM DTT, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 12 mg/ml was injected at a 0.20 ml/min flow rate onto a Shodex KW404-4F column at 20°C. 750 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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