S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.

Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV, J Biol Chem 300(9):107627 (2024) Europe PMC

SASDVN2 – S.aureus Protein Map Eap3 and Eap4 domains (Δ266-476)

Protein map
MWexperimental 20 kDa
MWexpected 24 kDa
VPorod 28 nm3
log I(s) 1.86×101 1.86×100 1.86×10-1 1.86×10-2
Protein map small angle scattering data  s, nm-1
ln I(s)
Protein map Guinier plot ln 1.86×101 Rg: 2.5 nm 0 (2.5 nm)-2 s2
(sRg)2I(s)/I(0)
Protein map Kratky plot 1.104 0 √3 sRg
p(r)
Protein map pair distance distribution function Rg: 2.6 nm 0 Dmax: 8.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Protein map MULTIFOXS model
log I(s)
 s, nm-1
Protein map MULTIFOXS model
Protein map MULTIFOXS model
log I(s)
 s, nm-1
Protein map MULTIFOXS model
Protein map MULTIFOXS model
Protein map MULTIFOXS model
Synchrotron SAXS data from solutions of S.aureus Protein Map Eap3 and Eap4 domains (Δ266-476) in 20 mM HEPES, 140 mM NaCl, pH 7.4 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 5 mg/ml was injected at a 0.65 ml/min flow rate onto a Shodex KW-803 column at 20°C. 660 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Protein map (Eap34)
Mol. type   Protein
Organism   Staphylococcus aureus (strain Mu50 / ATCC 700699)
Olig. state   Monomer
Mon. MW   24.1 kDa
 
UniProt   Q99QS1 (266-476)
Sequence   FASTA