The structure and function of the DNA binding domain of class B MpARF2 share more traits with class A AtARF5 than to that of class B AtARF1

SASDVV5 – Arabidopsis thaliana Auxin Response Factor 1 in complex with high affinity DNA

Auxin response factor 1
High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing
MWexperimental 94 kDa
MWexpected 94 kDa
VPorod 136 nm3
log I(s) 9.34×100 9.34×10-1 9.34×10-2 9.34×10-3
Auxin response factor 1 High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing small angle scattering data  s, nm-1
ln I(s)
Auxin response factor 1 High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing Guinier plot ln 9.34×100 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
Auxin response factor 1 High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing Kratky plot 1.104 0 3 sRg
p(r)
Auxin response factor 1 High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing pair distance distribution function Rg: 3.5 nm 0 Dmax: 10.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of A. thaliana auxin response factor 1 in complex with high affinity DNA in 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH 8 were collected on the BL11 - NCD beam line at ALBA (Cerdanyola del Vallès, Barcelona, Spain) using a ADSC Quantum 210r detector at a sample-detector distance of 2.6 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.50 mg/ml was measured at 20°C. 20 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

CAUTION: Dmax appears underestimated.

Auxin response factor 1 (AtARF1)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Dimer
Mon. MW   40.3 kDa
 
UniProt   Q8L7G0 (1-355)
Sequence   FASTA
 
High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing (HA7)
Mol. type   DNA
Olig. state   Dimer
Mon. MW   6.5 kDa
Sequence   FASTA