SASDVZ2 – Human Collagen Galactosyltransferase GLT25D1/COLGALT1

Procollagen galactosyltransferase 1

MW^I(0)	131	kDa
MW^expected	137	kDa
V^Porod	184	nm³

log I(s) 2.29×10¹ 2.29×10⁰ 2.29×10^-1 2.29×10^-2

Procollagen galactosyltransferase 1 small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Procollagen galactosyltransferase 1 Guinier plot

ln 2.29×10¹ R_g: 4.3 nm 0 (4.3 nm)^-2 s²

(sR_g)²I(s)/I(0)

Procollagen galactosyltransferase 1 Kratky plot

1.104 0 √3 sR_g

p(r)	0 D_max: 14.0 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.8

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.074
1.137

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Procollagen galactosyltransferase 1 CORAL model

Synchrotron SAXS data from solutions of Human Collagen Galactosyltransferase GLT25D1/COLGALT1 in 25 mM HEPES, 0.1 M NaCl, pH 8 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099187 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 4 mg/ml was injected at a 0.06 ml/min flow rate onto a GE Superdex 200 Increase 3.2/300 column at 20°C. 1200 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Procollagen galactosyltransferase 1 (GLT25D1)
Mol. type		Protein
Organism		Homo sapiens
Olig. state		Dimer
Mon. MW		68.5 kDa

UniProt		Q8NBJ5 (30-618)
Sequence		FASTA