Synchrotron SAXS
data from solutions of
Mitochondrial intermembrane space import and assembly protein 40 (CHCHD4) triple mutant I12A/F14A/H20A (AIA-A)
in
25 mM HEPES, 150 mM NaCl, 2 mM TCEP, pH 7.5
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a sample-detector distance of 2 m and
at a wavelength of λ = 0.127 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 7.30 mg/ml was measured
at 10°C.
33 successive
0.300 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Experimental molecular weight determined from SEC-MALS. Molecular weight by volume of correlation (Vc) is 19.8 kDa. Real-space PDDF I(0) should be 39.2 based on ScAtter P(r) calculation.
s, nm-1
