Synchrotron SAXS data from solutions of Immunoglobulin G in 100 mM glycine-HCl, 200 mM NaCl, pH 2 were collected on the BL-10C beam line at the Photon Factory High Energy Accelerator Research Organization (KEK; Tsukuba, Japan) using a Pilatus3 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 400.00 μl sample at 10 mg/ml was injected at a 0.20 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 25°C. 13 successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
DrugBank ID: DB00072 https://go.drugbank.com/drugs/DB00072. Heavy chains' C-terminal K residues are deleted for Herceptin (Chugai Pharmaceutical Co., Ltd., Tokyo, Japan), commercially available trastuzumab, according to the information in PMDA (Pharmaceuticals and Medical Devices Agency): Review report regarding the anti-HER2 humanized monoclonal antibody Herceptin® Injection 60 and 150, on Jan. 16th 2008 (https://www.pmda.go.jp/drugs/2007/P200700066/45004500_21300AMY00128_A100_2.pdf).
s, nm-1
