Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate

Henrik Vinther Sørensen.

SASDW72 – Non-deuterated N-acetylglucosamine binding Protein A (H-GbpA) in 47 % D₂O, 2.5 mg/mL

GlcNAc-binding protein A
MWexpected 51 kDa
log I(s) 6.14×10-1 6.14×10-2 6.14×10-3 6.14×10-4
GlcNAc-binding protein A small angle scattering data  s, nm-1
ln I(s)
GlcNAc-binding protein A Guinier plot ln 6.14×10-1 Rg: 25.0 nm 0 (25.0 nm)-2 s2
(sRg)2I(s)/I(0)
GlcNAc-binding protein A Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

SANS data from solutions of non-deuterated N-acetylglucosamine binding Protein A (H-GbpA) in 100 mM NaCl, 20 mM Tris, 47% v/v D₂O, pH 8 were collected on the D11 beam line at the Institut Laue-Langevin (ILL; Grenoble, France). Data recorded as I(s) vs s (where s = 4πsinθ/λ, and 2θ is the scattering angle), were collected at a sample concentration of 2.5 mg/mL at 25 degrees centigrade using three instrument configurations: Set-up 1: Sample-Detector distance, 1.7 m; Collimation distance, 5.5 m; Wavelength, 0.56 nm; Frames, 1; Time per frame, 900 s. Set-up 2: Sample-Detector distance, 8.0 m; Collimation distance, 8.0 m; Wavelength, 0.56 nm; Frames, 1; Time per frame, 900 s. Set-up 3: Sample-Detector distance, 38.95 m; Collimation distance, 40.0 m; Wavelength, 0.56 nm; Frames, 1; Time per frame, 900 s.

Guinier analysis and subsequent Rg, I(0) and MW parameters are inaccurate for this entry as the protein is contrast matched.

GlcNAc-binding protein A (H-GbpA)
Mol. type   Protein
Organism   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Olig. state   Monomer
Mon. MW   51.3 kDa
 
UniProt   Q9KLD5 (24-485)
Sequence   FASTA