Early events in G-quadruplex folding captured by time-resolved small-angle X-ray scattering.

Monsen RC, Sabo TM, Gray R, Hopkins JB, Chaires JB, Nucleic Acids Res 53(3) (2025) Europe PMC

SASDW83 – Telomere DNA G-quadruplex (2GKU) at alkaline pH

Telomere DNA G-quadruplex Hybrid-1 form
MWexperimental 6 kDa
MWexpected 8 kDa
VPorod 12 nm3
log I(s) 1.77×10-2 1.77×10-3 1.77×10-4 1.77×10-5
Telomere DNA G-quadruplex Hybrid-1 form small angle scattering data  s, nm-1
ln I(s)
Telomere DNA G-quadruplex Hybrid-1 form Guinier plot ln 1.78×10-2 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
Telomere DNA G-quadruplex Hybrid-1 form Kratky plot 1.104 0 √3 sRg
p(r)
Telomere DNA G-quadruplex Hybrid-1 form pair distance distribution function Rg: 2.5 nm 0 Dmax: 8.3 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Telomere DNA G-quadruplex (2GKU) in 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH 11.5, pH 11.5 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 250.00 μl sample at 7.1 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 22°C. Two successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Telomere DNA G-quadruplex Hybrid-1 form (2GKU)
Mol. type   DNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   7.7 kDa
Sequence   FASTA