Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate

Henrik Vinther Sørensen.

SASDWB2 – 3 mg/mL β-chitin nanofibers from squid pens with 2.5 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O - USANS

β-chitin nanofibers from squid pens
GlcNAc-binding protein A (perdeuterated)
MWexpected 51 kDa
log I(s)
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) small angle scattering data  s, nm-1
s2I(s)
β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) Kratky plot 0 s

Data validation


There are no models related to this curve.

Ultra small angle neutron scattering (USANS) data from of β-chitin nanofibers from squid pens mixed with perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 20 mM acetate, 47% v/v D₂O, pH 5 were collected on the BT5 USANS instrument at the National Institute of Standards and Technology (NIST; Gaithersburg, MD, United States). Data recorded as I(s) vs s (where s = 4πsinθ/λ, and 2θ is the scattering angle), were collected at a sample concentration of 3 mg/mL chitin and 2.5 mg/mL D-GbpA at 25 degrees centigrade using the following instrument configuration: Sample-Detector distance, 30 m; Wavelength, 0.24 nm; Frames, 1; Time per frame, 11 hours; s-range, 0.0005 nm-1 to 0.01 nm-1.

Guinier analysis and subsequent Rg, I(0) and MW parameters are not applicable for this entry as the Guinier-region occurs at a much lower s-range than the data includes. The chitin fibers are micrometers long.

β-chitin nanofibers from squid pens (β-chitin)
Mol. type   Other
Organism   Squid
Mon. MW   0.2 kDa
Chemical formula
 
GlcNAc-binding protein A (perdeuterated) (D-GbpA)
Mol. type   Protein
Organism   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Olig. state   Monomer
Mon. MW   51.3 kDa
 
UniProt   Q9KLD5 (24-485)
Sequence   FASTA