Synchrotron SAXS
data from solutions of
Proliferating cell nuclear antigen:PCNA-associated factor (PCNA:P15) titration, 150 µM:300 µM
in
137 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 2 mM potassium phosphate (PBS), pH 7
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.3 and 20 mg/ml were measured
at 25°C.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The Rg and the MW are not properly estimated as the curve has been truncated to avoid interparticle interactions. This is a profile from a titration series.
s, nm-1
