| MWI(0) | 53 | kDa |
| MWexpected | 50 | kDa |
| VPorod | 74 | nm3 |
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log I(s)
9.22×101
9.22×100
9.22×10-1
9.22×10-2
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s, nm-1
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Small-angle X-ray scattering of engineered antigen-binding fragments: the case of glycosylated Fab from the Mannitou IgM antibody.
Semwal S, Karamolegkou M, Flament S, Raouraoua N, Verstraete K, Thureau A, Wien F, Bray F, Savvides SN, Bouckaert J, Acta Crystallogr F Struct Biol Commun (2025) Europe PMCSASDWK2 – Mannitou IgM Fab Monomer
IgM Mannitou Fab Heavy ChainIgM Mannitou Fab Light Chain
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Fits and models
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Synchrotron SAXS data from solutions of mannitou IgM Fab in 20 mM HEPES, 300 mM NaCl, pH 7.5 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 9.2 mg/ml was injected at a 0.30 ml/min flow rate onto a Agilent Bio SEC-3, 300 Å column at 15°C. 580 successive 0.990 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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