| MWexperimental | 18 | kDa |
| MWexpected | 13 | kDa |
| VPorod | 30 | nm3 |
|
log I(s)
1.77×10-2
1.77×10-3
1.77×10-4
1.77×10-5
|
s, nm-1
|
Extremophilic hemoglobins: The structure of Shewanella benthica truncated hemoglobin N
Martinez Grundman J, Schultz T, Schlessman J, Johnson E, Gillilan R, Lecomte J, Journal of Biological Chemistry :108223 (2025) DOISASDWK4 – Shewanella benthica hemoglobin
Group 1 truncated hemoglobin (C51S, C71S)|
|
|
Fits and models
|
|
|
Synchrotron SAXS
data from solutions of
Shewanella benthica hemoglobin
in
14 mM Tris, 6 mM potassium phosphate, pH 7
were collected
on the
ID7A1 BioSAXS / HP-Bio Beamline beam line
at the Cornell High Energy Synchrotron Source (CHESS) storage ring
(Ithaca, NY, USA)
using a Eiger 4M detector
at a sample-detector distance of 1.6 m and
at a wavelength of λ = 0.1241 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample
at 10 mg/ml was injected at a 0.50 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 23°C.
One
2 second frame was collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
40% monomer + 60% dimer. Single 2-sec exposure from peak of SAXS chromatogram. Fit to monomer and dimer from PDB: 7TT9 via OLIGOMER. |
|
|||||||||||||||||||||||||||||||||||||||