Synchrotron SAXS
data from solutions of
Shewanella benthica hemoglobin 0.101 MPa (final measurement after end of pressure series)
in
14 mM Tris, 6 mM potassium phosphate, pH 7
were collected
on the
ID7A1 BioSAXS / HP-Bio Beamline beam line
at the Cornell High Energy Synchrotron Source (CHESS) storage ring
(Ithaca, NY, USA)
using a Eiger 4M detector
at a sample-detector distance of 1.8 m and
at a wavelength of λ = 0.0883 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 10.00 mg/ml was measured
at 23°C.
Four successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Final measurement returned to 0.101 MPa (1 standard atmosphere) after pressure series.
s, nm-1
