Structural and molecular comparison of bacterial and eukaryotic trigger factors.

Ries F, Carius Y, Rohr M, Gries K, Keller S, Lancaster CRD, Willmund F, Sci Rep 7(1):10680 (2017) Europe PMC

SASDX32 – chloroplast trigger factor from Arabidopsis thaliana

Trigger factor-like protein TIG, Chloroplastic
MWexperimental 51 kDa
MWexpected 53 kDa
VPorod 104 nm3
log I(s) 4.96×101 4.96×100 4.96×10-1 4.96×10-2
Trigger factor-like protein TIG, Chloroplastic small angle scattering data  s, nm-1
ln I(s)
Trigger factor-like protein TIG, Chloroplastic Guinier plot ln 4.96×101 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Trigger factor-like protein TIG, Chloroplastic Kratky plot 1.104 0 √3 sRg
p(r)
Trigger factor-like protein TIG, Chloroplastic pair distance distribution function Rg: 3.8 nm 0 Dmax: 12.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Trigger factor-like protein TIG, Chloroplastic DAMMIN model
Synchrotron SAXS data from solutions of chloroplast trigger factor from Arabidopsis thaliana in 20 mM Tris pH 7.5, 150 mM KCl were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 8 mg/ml were measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Trigger factor-like protein TIG, Chloroplastic (AtTIG1)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Monomer
Mon. MW   53.2 kDa
 
UniProt   Q8S9L5 (78-547)
Sequence   FASTA