Arrestin-3 fused to extracellular signal-regulated kinase 2

Arrestin-3-ERK2-His1 protein

Organism: Bos taurus/Rattus norvegicus

Monomeric molecular weight: 88.9 kDa

Oligomeric state: Dimer

Total molecular weight: 177.8 kDa

Sequence:

MGEKPGTRVFKKSSPNCKLTVYLGKRDFVDHLDKVDPVDGVVLVDPDYLKDRKVFVTLTCAFRYGREDLDVLGLSFRKDLFIANYQAFPPTPNPPRPPTRLQERLLRKLGQHAHPFFFTIPQNLPCSVTLQPGPEDTGKACGVDFEIRAFCAKSLEEKSHKRNSVRLVIRKVQFAPEKPGPQPSAETTRHFLMSDRSLHLEASLDKELYYHGEPLNVNVHVTNNSTKTVKKIKVSVRQYADICLFSTAQYKCPVAQVEQDDQVSPSSTFCKVYTITPLLSNNREKRGLALDGKLKHEDTNLASSTIVKEGANKEVLGILVSYRVKVKLVVSRGGDVSVELPFVLMHPKPHDHIALPRPQSAVPETDAPVDTNLIEFETNYATDDDIVFEDFARSGLEVLFQGPGSMAAAAAAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNLNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRSGSLVPRGSGSHHHHHHHHHH

In brief, the construct was cloned into a pTrc vector as follows: bovine arrestin-2 or -3 cDNA, linker with TEV cleavage site (SGLEVLFQGPGS), rat ERK2 cDNA, thrombin cleavage site (GSLVPRGSGS), and 10X His-tag. For arrestin-3: UniProt Accession #: P32120-2 (ARRB2 – BOVIN) Resides 1-393 Bovine arrestin-3 isoform short MW: 44.17 for monomer Arrestin-3 mediates desensitization and internalization of G protein-coupled receptors. For ERK2: UniProt Accession #: P63086-1 (MK01_RAT) Residues 1-358 Rat mitogen-activated protein kinase 1 Mapk1/ERK2 MW: 41.28 for monomer Known to dimerize Extracellular signal-regulated kinase 2 (ERK2) is a serine/threonine kinase that functions in cell growth, survival, and differentiation. It is a member of the Raf/MEK1/ERK kinase cascade.