TnpA transposase

TnpA WT protein

Organism: Bacillus thuringiensis serovar thuringiensis str. IS5056

Monomeric molecular weight: 116.8 kDa

Oligomeric state: Dimer

Total molecular weight: 233.5 kDa

UniProt: M1QZ58 (1-987)

Sequence:

MGVKQLLSEAQRNELMDLSRLTEWDLVTFHTFSKHDLHLILKHRRGYNRLGFALQLVLIR YPGWSLTEYKDIPQYVVAYVASQLQIPPEEFLVYAKRGNTLWEHLGEIRTEYGYQNFSSE YKETLLQFLVQQAMDNNNTLYLIEITISTLRKMKVILPAMYVIEDIVWEAKQQADQKVYS ILHDGLVQEQKDQLDALLLPTINGKSPLAWLKDVPAQPSPESFLKVIDRLQFVQKIGLTI DTTKINTNRLRQLARLGSKYEPYAFRRFNEVKRYSMLVSFLLEITQDLIDYAIEIHDRLM MNLQTKGKKEQDEIQQANGKKLNEKILQFITVCGTLIEAKETGKDAFAALDEVMSWNEMV ESVEEAKQLSRPLNYDYLDLLNTRYSYVRRYAPTLLRSLHFRATKSGEPVLQALDTIHEL NETGKRKVPHGAPLHFVSNRWQKHVYDDDGNINRHYYELAALTELRNHIRSGDIFVSGSR HHKAFDDYLIPYDEWNEVSNIPNGLTAPLKAEDYITDRINRLNEHLEWLSKNSEKLEGVD ISQGKLHVERLDRGTPEEAKAFSKLLHSMLPRIKLTDLLIEVASWTGFHDQFIHASTNQS PDQEEQNIVLATLMAMGTNIGLTKMAEATPGISYRQMANASQWRMYDDAMVRAQSILVNF QKEQKLSSYWGDGTTSSSDGMRLSIAVRSLHADSNPHYGTGKGGTIYRFVSDQLSAYHVK VITTNARDALHVLDGLLHHETDLKIEEHYTDTAGYTDQVFALTHLLGFRFAPRIRDLADT KLFSIPGGEEYENVQALLKGKINVKLIKENYEDIRRLAYSVQTGKVSSALIMGKLGSYAR QNKLATALGEMGRIEKTLFTLDYISNKAVRRRVQKGLNKGEAINALARIIFFGQRGEFRE RALQDQLQRASALNIIINAISVWNTVYMEKAVEELKARGEFREDLMPYAWPLGWEHINFL GEYKFEGLHDTGQMNLRPLRIKEPFYS PIRSFLEQKLISEEDLNSAVDHHHHHH

TnpA WT with a cMyc-His6 at the C-terminus.