GSHHHHHHGSLRAAIRKPGKVGPPLDIKLGALNCTAFSIQWKMPRHPGSPILGYTVFYSEVGADKSLQEQ LHSVPLSRDIPTTEEVIGDLKPGTEYRVSIAAYSQAGKGRLSSPRHVTTLSQDSCLPPAA PQQPHVIVVSDSEVALSWKPGASEGSAPIQYYSVEFIRPDFDKKWTSIHERIQMDSMVIK GLDPDTNYQFAVRAMNSHGPSPRSWPSDIIRTLAAA
Recombinant Pika-FnIII(1-2) was produced using HEK293-F cells with an N-terminal Histidine tag. Purification was carried out using IMAC followed by Gel filtration in 25 mM HEPES/NaOH, 200 mM NaCl, pH 8