GSAAHPCVRAPCAHGGSCRPRKEGYDCDCPLGFEGLHCQKECGNYCLNTIIEAIEIPQFIGR SYLTYDNPDILKRVSGSRSNVFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVF SYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGA LYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGAKAAA
Recombinant Pika-EGF-LG3 was produced using HEK293-F cells. The construct was produced using a cleavable N-terminal His-tag. The tag was removed after IMAC. The purified sample was then subject to gel filtration prior to SEC-SAXS analysis.