Accumulation associated protein (mutant)

Aap protein

Organism: Staphylococcus epidermidis (strain ATCC 35984 / RP62A)

Monomeric molecular weight: 77.7 kDa

Oligomeric state: Dimer

Total molecular weight: 155.4 kDa

UniProt: Q5HKE8 (1505-2223)

Sequence:

GVDGDPITSTEEIPFDKKREFNPDLKPGEERVKQKGEPGTKTITTPTTKNPLTGEKVGEGEPTEKITKQPVDEITEYGGE EIKPGAKDEFDPNAPKGSQEDVPGKPGVKNPDTGEVVTPPVDDVTKYGPVDGDPITSTEEIPFDKKREFDPNLAPGTEKV VQKGEPGTKTITTPTTKNPLTGEKVGEGEPTEKITKQPVDEITEYGGEEIKPGAKDEFDPNAPKGSQEDVPGKPGVKNPD TGEVVTPPVDDVTKYGPVDGDPITSTEEIPFDKKREFDPNLAPGTEKVVQKGEPGTKTITTPTTKNPLTGEKVGEGEPTE KITKQPVDEIVHYGGEEIKTGAKDEFDPNAPKGSQTTQPGKPGVKNPDTGEVVTPPVDDVTKYGPVDGDPITSTEEIPFD KKREFDPNLAPGTEKVVQKGEPGTKTITTPTTKNPLTGEKVGEGEPTEKITKQPVDEIVHYGGEEIKPGAKDEFDPNAPK GSQEDVPGKPGVKNPDTGEVVTPPVDDVTKYGPVDGDSITSTEEIPFDKKREFDPNLAPGTEKVVQKGEPGTKTITTPTT KNPLTGEKVGEGEPTEKITKQPVDEIVHYGGEQIPQGAKDEFDPNAPVDSKTEVPGKPGVKNPDTGEVVTPPVDDVTKYG PVDGDSITSTEEIPFDKKREFDPNLAPGTEKVVQKGEPGTKTITTPTTKNPLTGEKVGEGKSTEKVTKQPVDEIVEYGPT GENLYFQ

Brpt5.5 5xH85A (G5 08 - G5 13) in the presence of 5 mM ZnCl2, which induces dimerization. This is a mutant construct which contains a His to Ala mutation in the 85 position of each spacer subdomain.