Accumulation associated protein (mutant)

Aap protein

Organism: Staphylococcus epidermidis (strain ATCC 35984 / RP62A)

Monomeric molecular weight: 77.9 kDa

Oligomeric state: Tetramer

Total molecular weight: 311.6 kDa

UniProt: Q5HKE8 (1505-2223)

Sequence:

GVDGDPITSTEEIPFDKKREFNPDLKPGEERVKQKGEPGTKTITTPTTKNPLTGEKVGEGEPTEKITKQPVDEITEYGGE EIKPGHKDEFDPNAPKGSQEDVPGKPGVKNPDTGEVVTPPVDDVTKYGPVDGDPITSTEEIPFDKKREFDPNLAPGTEKV VQKGEPGTKTITTPTTKNPLTGEKVGEGEPTEKITKQPVDEITEYGGEEIKPGAKDEFDPNAPKGSQEDVPGKPGVKNPD TGEVVTPPVDDVTKYGPVDGDPITSTEEIPFDKKREFDPNLAPGTEKVVQKGEPGTKTITTPTTKNPLTGEKVGEGEPTE KITKQPVDEIVHYGGEEIKTGHKDEFDPNAPKGSQTTQPGKPGVKNPDTGEVVTPPVDDVTKYGPVDGDPITSTEEIPFD KKREFDPNLAPGTEKVVQKGEPGTKTITTPTTKNPLTGEKVGEGEPTEKITKQPVDEIVHYGGEEIKPGAKDEFDPNAPK GSQEDVPGKPGVKNPDTGEVVTPPVDDVTKYGPVDGDSITSTEEIPFDKKREFDPNLAPGTEKVVQKGEPGTKTITTPTT KNPLTGEKVGEGEPTEKITKQPVDEIVHYGGEQIPQGHKDEFDPNAPVDSKTEVPGKPGVKNPDTGEVVTPPVDDVTKYG PVDGDSITSTEEIPFDKKREFDPNLAPGTEKVVQKGEPGTKTITTPTTKNPLTGEKVGEGKSTEKVTKQPVDEIVEYGPT GENLYFQ

Brpt5.5 2xH85A (G5 08 - G5 13) in the presence of 5 mM ZnCl2, which induces tetramerization. This is a mutant construct which contains a His to Ala mutation in the 85 position of spacer subdomains 09 and 11 (H213A, H469A).