HOTag-(PA)25-Ubiquitin

Organism: synthetic construct

Monomeric molecular weight: 16.9 kDa

Oligomeric state: Tetramer

Total molecular weight: 67.5 kDa

UniProt: P0CG47 (1-76)

Sequence:

MTLREIEELLRKIIEDSVRSVAELEDIEKWLKKIPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

HOTag6 is a ~30-amino-acid tetramerization domain followed by a (Pro-Ala)25 linker followed by the sequence for human monoubiquitin (UniProt ID: P0CG47, https://www.uniprot.org/uniprot/P0CG47, amino acids 1-76) . This is a designed protein (no natural variant exists). The HOTag6 sequence is from the Shu group at the University of California, San Francisco (https://doi.org/10.1016/j.molcel.2017.12.008).