Chimeric construct containing the GST-like domains of P. berghei tRNA import protein and glutamyl-tRNA synthetase

tRip-N-ERS-N protein

Organism: Plasmodium berghei ANKA

Monomeric molecular weight: 52.6 kDa

Oligomeric state: Dimer

Total molecular weight: 105.1 kDa

Sequence:

MGCTLNLVENDIKSDILKLALDFIKTNIVENDDNVVFPEIKYGQKISYEHNKKTYKEFFC SLYAIIDTYNCYSQFFCENEDKVSESEEFIFNLASDKFKLKPLDMKHLNDILRERSYIVS DKHASLVDIFYFCCVYKILKPMTAKEKVEYYHICRWYIHLQETLICDFVKLHKLDIRSGV ESLLNSGIVTCTNEKASNEQVGSPLVMIKDSKINIYYGKNYPFLCRTVFNIYQNNIKKKT ANNLNDKKIKEICVNFINDKTVVEDIKVEFVRNTVSDQTRKNTNTNNSVTSSDKIFAINL DFLLKTNLYYFTSYRENINRNIITNVFFQAQYNEWIDFLRNKDIEKNIIPICEHINKHLY LNTFLSFHYLTLSDIYIYYEMHKYFSGNITTNLKYPKQYKNINRWFRLIKALLHDHVATD AELIQNLKVKEKIFIDGGSSGLVPR

Chimeric protein containing the GST-like domains of P. berghei tRNA import protein (A0A509ARF0, residues 1-202) and glutamyl-tRNA synthetase (A0A509AR09, residues 1-228). Additional amino acids at the C-terminus resulted from the cleavage of the 6xHis tag. The construction displays a glycine insertion after the first methionine derived from the cloning restriction site.