DNA-guanine transglycosylase - D95A mutant

DpdA(D95A) protein

Organism: Salmonella enterica subsp. enterica serovar Montevideo

Monomeric molecular weight: 50.4 kDa

Oligomeric state: Monomer

Total molecular weight: 50.4 kDa

UniProt: P0DW33 (2-416)

Sequence:

HHHHHHSSGLVPRGSHMHMASLSKLKYFFPDSQDFIDPSFDFVRETRNEHRVRQRDDHYPHEVFPHPYDGMLVSKAVVDGLGGGESKYTRAQRLRYFRNGMKHFFRLPDNMQTMGACGAFTYVNQDVPPYRVEEVIEFYETSRFNYGVSLDHIIFGYEKPGESFSGEVLAECRRRQDITLTLAQDFLVKSQKSCFTPFGVAHGWNKKSYRQSVEALLAMGYKNITMGGMVPLKTAQILETLEEIKPLLKSDTQVHLLGIARPESFADFIRLGVTSIDSTTPLQQAFKDRKNNYHTPEGRAYTAVRVPQFDANPSLSRKIKSGVIDQDVARHLEKDAMHALFEYDNNALSLEKTLEAVLAYERLHSGEKEAEKIRADYERTLGDRPWRKCECNICRSIGINVIIFRGAERNRRRGFHNIQVLYNRLQYTLSLRSEDKS

7-deazapurine in DNA protein A D95A mutant. D95A is a catalytically dead mutant of DpdA. The protein construct used for SAXS contains an N-terminal polyhistidine tag.