Netrin-1

NET1 ΔC protein

Organism: Gallus gallus

Monomeric molecular weight: 49.5 kDa

Oligomeric state: Pentamer

Total molecular weight: 247.5 kDa

UniProt: Q90922 (26-458)

Sequence:

APLAGYPGLNMFAVQTAQPDPCYDEHGLPRRCIPDFVNSAFGKEVKVSSTCGKPPSRYCV VTEKGEEQVRSCHLCNASDPKRAHPPSFLTDLNNPHNLTCWQSDSYVQYPHNVTLTLSLG KKFEVTYVSLQFCSPRPESMAIYKSMDYGKTWVPFQFYSTQCRKMYNKPSRAAITKQNEQ EAICTDSHTDVRPLSGGLIAFSTLDGRPTAHDFDNSPVLQDWVTATDIKVTFSRLHTFGD ENEDDSELARDSYFYAVSDLQVGGRCKCNGHASRCVRDRDDNLVCDCKHNTAGPECDRCK PFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRH CHYCKEGFYRDLSKPISHRKACKECDCHPVGAAGQTCNQTTGQCPCKDGVTGITCNRCAK GYQQSRSPIAPCIKIPAGSLVPR

Netrin-1 ΔC is an extracellular matrix glycoprotein. It was expressed in human embryonic kidney 293T cells and contain 3 N-linked glycosylation sites. Assuming the presence of 3 core pentasaccharides, the formula mass of the monomer would be 52.4 kDa. The expression construct encompasses the Laminin N-terminal (LN, VI) domain, Laminin EGF-like domain 1 (LE1, Vᵢ), Laminin EGF-like domain 2 (LE2, Vᵢᵢ) and Laminin EGF-like domain 3 (LE3, Vᵢᵢᵢ). The C-terminal NTR domain is missing. In this scattering profile, the most probable number of Netrin-1 ΔC molecules associated to heparin oligosaccharide dp10 is 5 or 6; refer to the associated publication for more details.