Gag-Pol polyprotein (588-1147: W988A mutant; Reverse transcriptase/ribonuclease H; p66)

p66(W401A) protein

Organism: Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)

Monomeric molecular weight: 66.5 kDa

Oligomeric state: Monomer

Total molecular weight: 66.5 kDa

UniProt: P04585 (588-1147)

Sequence:

MNHKVHHHHHHLEGRHMPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAELELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGAHTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETAWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNRGRQKVVTLTDTTNQKTELQAIYLALQDSGLEVNIVTDSQYALGIIQAQPDQSESELVNQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDKLVSAGIRKVL

W401A mutant of p66 (W988A mutant of Gag-Pol polyprotein). The protein construct used for SAXS contains a N-terminal polyhistidine tag.