Gag-Pol polyprotein (588-1027: W988A mutant; Reverse transcriptase p51; p51 RT)

p51(401A) protein

Organism: Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)

Monomeric molecular weight: 53.4 kDa

Oligomeric state: Monomer

Total molecular weight: 53.4 kDa

UniProt: P04585 (588-1027)

Sequence:

MNHKVHHHHHHLEGRHMPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAELELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGAHTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETAWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIVGAETF

W401A mutant of p51 RT (W988A mutant of Gag-Pol polyprotein). Proteolytic cleavage of p66 removes the RNase H domain to yield the p51 RT subunit. The protein construct used for SAXS contains a N-terminal polyhistidine tag.