Acylamino-acid-releasing enzyme (I277L, V491A)

S9Cgs protein

Organism: Geobacillus stearothermophilus

Monomeric molecular weight: 77.1 kDa

Oligomeric state: Tetramer

Total molecular weight: 308.4 kDa

UniProt: A0A150M835 (17-670)

Sequence:

MASWSHPQFEKGSSHHHHHHSSGSGGGGGENLYFQGTDLLRLRSVRDPHYAPDGTRAVFVEKSIDEEKQYRSHLWIWAADGSVRQWTFGRWRDMKPRFSPRGEIIAFLSDRSGRTQLWLLPANGGEARQLTFFKNGVRDYVWSPDGTFLITLTTLGDDETIEDREEPEKVEVKRADLKPRVVERLYYKSDASGFLDGKRAVLTRIDVLSGKSEALTGREEEIGSFAISPNGRTLAFVANRNEDPDTTFTRDIVLLDLESKAETNLTNGCGTFASLAWSPDGTKLAAIGHDLAYLGATLHRLYVFEPERGTKRVLTADWDVHLGDAMVGDTHADAKGPGPIWASDGSGLYVTASERGRVNLYFVSLAGPIVPVIEGNFHLYGLAIHPSEQQAIAAISSPTSVGDLYAVSLADGTKTRLTRANEALENEVVFADAEPFTYRSADGLEIQGWIMKPPELDEGEKAPLVVEIHGGPHAMYGFTFFHELQLLASSGYAVLFTNPRGSHGYGQSFVNAVRGDYGGMDYEDIMAGVDAAISKFDFIDKERLGVTGGSYGGFMTNWIVGHTDRFKAAVTQRSISNWLSFSGVSDIGYFFTKWEVGCDVWEDAERLWHHSPLKYVKHMRTPLLILHSERDYRCPIEQAEQLFVALKQLGRETKLVRFPDANHDLSRTGNPALRLERLRHIVDWFDRYLKG

The protein construct used for SAXS contains and additional N-terminal MASWSHPQFEKGSSHHHHHHSSGSGGGGGENLYFQGT amino acid extension and two point mutations (I277L, V491A) relative to the native sequence in UniProt.