Hepatocyte nuclear factor 1-alpha

HNF-1A DBD-TAD protein

Organism: Homo sapiens

Monomeric molecular weight: 62.2 kDa

Oligomeric state: Monomer

Total molecular weight: 62.2 kDa

UniProt: P20823 (83-631)

Sequence:

MGPHHHHHHLESTSLYKKAGSENLYFQGPPILKELENLSPEEAAHQKAVVETLLQEDPWRVAKMVK SYLQQHNIPQREVVDTTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREVAQQFTHA GQGGLIEEPTGDELPTKKGRRNRFKWGPASQQILFQAYERQKNPSKEERETLVEECNRAE CIQRGVSPSQAQGLGSNLVTEVRVYNWFANRRKEEAFRHKLAMDTYSGPPPGPGPGPALP AHSSPGLPPPALSPSKVHGVRYGQPATSETAEVPSSSGGPLVTVSTPLHQVSPTGLEPSH SLLSTEAKLVSAAGGPLPPVSTLTALHSLEQTSPGLNQQPQNLIMASLPGVMTIGPGEPA SLGPTFTNTGASTLVIGLASTQAQSVPVINSMGSSLTTLQPVQFSQPLHPSYQQPLMPPV QSHVTQSPFMATMAQLQSPHALYSHKPEVAQYTHTGLLPQTMLITDTTNLSALASLTPTK QVFTSDTEASSESGLHTPASQATTLHVPSQDPAGIQHLQPAHRLSASPTVSSSSLVLYQS SDSSNGQSHLLPSNHSVIETFISTQMASSSQ

HNF-1A DNA-binding domain and transactivation domain (DBD-TAD). The protein construct used for SAXS contains an additional N-terminal polyhistidine tag (His6x tag with linker and TEV protease cleavage site).