LPXTG-motif cell wall anchor domain protein

LrpA protein

Organism: Ligilactobacillus ruminis ATCC 25644

Monomeric molecular weight: 49.0 kDa

Oligomeric state: Monomer

Total molecular weight: 49.0 kDa

UniProt: E7FRT5 (35-472)

Sequence:

MGRDPNSETSTETATVTLHKYVFDKSLPSDKIDNSKSQDEINAWLTKNNAE ALDGVEFTAYDVTSEYADAYKTATGDKNESPADAAKTASAAVAKKADALQKT ATVVGKQTTANGGLASFANLPLRDANGNYKAYLFAETDAPANITQKAEPFV LAMPIYGADGKTVQKSINIYPKNVKQSDKKTLNDNRSHHDFTAGEKINYSIE TVVPWNIANKKVYTITDNPSKGLIMDADTIQIEGLASNKYTVKKNADNGFTIT IPAANLAAFAGKTLKTTVKGHLSIEDLTLIDTGIPNKATAKVDNEAHHEVKSEE VFTGGKKFVKVDGSNQSKTLAGAQFQLVIVKNGQVVKYAHGNEKDGYTFD TNNTNVATKTTGENGQFEFAGLKYSESLEAGESYAVKEVKAPTGYDLLKDPV LFTVTKDSYKTVQAADGQKISNTKKGGFLPLEHHHHHH

LrpA backbone-Pilin Subunit from Ligilactobacillus ruminis. The protein construct used for SAXS contains a C-terminal polyhistidine tag (LEHHHHHH) and an additional non-native amino acid sequence at the N-terminus (MGRDPNS).