SAM riboswitch element A aptamer domain (RNA)

SreA AD RNA

Organism: Listeria monocytogenes

Monomeric molecular weight: 39.1 kDa

Oligomeric state: Monomer

Total molecular weight: 39.1 kDa

Sequence:

GGUCUUAUCCAGAGUGGUGGAGGGAAAUGCCCUAUGAAGCCCAGCAACCUAAACAAUAAUUCAUUAUGUGUUUAAGGUGCUAAGUCAUGCAGAACAACUAAUUGUUCUGAAAGAUGAGACC

The SAM riboswitch element A sequence from Listeria monocytogenes was truncated to the conserved aptamer domain. Two non-native G residues were added to the 5' end of the molecule to facilitate in vitro transcription of the RNA with T7 RNA polymerase. Two non-native C residues were included at the 3' end of the molecule to form two stabilizing G-C base pairs at the bottom of the P1 stem.