Bacterial non-heme ferritin (N19Q, I59V)

FerHis protein

Organism: Helicobacter pylori (strain J99 / ATCC 700824)

Monomeric molecular weight: 20.0 kDa

Oligomeric state: 24-mer

Total molecular weight: 480.2 kDa

UniProt: Q9ZLI1 (5-167)

Sequence:

MHHHHHHGGSDIIKLLNEQVNKEMQSSNLYMSMSSWCYTHSLDGAGLFLFDHAAEEYEHAKKLIVFLNENNVPVQLTSISAPEHKFEGLTQIFQKAYEHEQHISESINNIVDHAIKSKDHATFNFLQWYVAEQHEEEVLFKDILDKIELIGNENHGLYLADQYVKGIAKSRKS

The protein construct used for SAXS contains an N-terminal polyhistidine tag (MHHHHHHGGS) and point mutations N19Q, I59V relative to UniProt Q9ZLI1.