Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion)

Fe8×FerSUMO protein
Organism: Helicobacter pylori (strain J99 / ATCC 700824)
Monomeric molecular weight: 31.5 kDa
Oligomeric state: Octamer
Total molecular weight: 251.8 kDa
UniProt: Q9ZLI1 (5-167)
Sequence:

The protein used for SAXS contains an N-terminal polyhistidine-SUMO tag (Ubiquitin-like protein SMT3 UniProt Q12306, amino acids 3-98; R64T, R71E) that span amino acids 1-111 of the SAXS construct. The bacterial non-heme ferritin protein begins at position 112 of the SAXS construct and spans amino acids 5-167 of the canonical amino acid sequence of the protein with two point mutations N19Q + I59V (numbering relative to UniProt Q9ZLI1).