Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S )

UP1 dm protein

Organism: Homo sapiens

Monomeric molecular weight: 24.9 kDa

Oligomeric state: Monomer

Total molecular weight: 24.9 kDa

UniProt: P09651 (1-196)

Sequence:

MHHHHHHSSGVDLGTENYLFQSNAMSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDSVVMRDPNTKRSRGFGFVTYATVEEVDAAMNADPHKVDGRVVEPKDAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNSEVRKALSKQEMASASSSQRGR

The protein construct used for SAXS contains an N-terminal polyhistidine tag (MHHHHHHSSGVDLGTENYLFQSNA). The N-terminal RNA recognition motifs of hnRNP A1 with two arginines on RRM1 (R75/R88) have been mutated to aspartate in addition to cysteine to serine mutations at positions 43 and 175.