Protein-ADP-ribose hydrolase (D13G, Y23S, T61A, I114S, R177H, I246T)

SpyMacro protein

Organism: Streptococcus pyogenes serotype M5 (strain Manfredo)

Monomeric molecular weight: 30.4 kDa

Oligomeric state: Monomer

Total molecular weight: 30.4 kDa

UniProt: P0DN70 (1-270)

Sequence:

GPMSMPSSFDLLGEMIGLLQTEQLTSSWACPLPNALTKRQDLWRALINQRPALPLSKDYLNLEDAYLDDWRASFVPVSVKDCQKTNYTSLFLYHGDIRYLAVDAIVNAANSELLGCFSPNHGCIDNAIHTFAGSRLRLACQAIMTEQGRKEAIGQAKLTSAYHLPASYIIHTVGPRITKGHHVSPIRADLLARCYRSSLDLAVKAGLTSLAFCSISTGEFGFPKKEAAQIAIKTVLKWQAEHPESKTLTTIFNTFTSEDKALYDTYLQKENNCE

The protein construct used for SAXS contains an additional four non-native amino acids at the N-terminus (GPMS).