Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A)

MBP protein

Organism: Escherichia coli (strain K12)

Monomeric molecular weight: 40.2 kDa

Oligomeric state: Dimer

Total molecular weight: 80.5 kDa

UniProt: P0AEX9 (27-384)

Sequence:

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDI IFWAHDRFGGYAQSGLLAEITPAAAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNK DLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYAAGKYDIK DVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSA VNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL GAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDA ALAAAQTNAAA

The complete form of the protein of interest is a fusion protein between the Escherichia coli Maltose-Binding Protein (MBP) (Uniprot: P0AEX9) and the Mycobacterium tuberculsosis MabR (Rv2242) (Uniprot: P9WPH5). The MBP used is a variant with an additional linker and point mutations (D108A, K109A, E198A, N199A, K265A). The fusion protein adopts three forms in solution: tetramer, dimer and monomer. Then, in SEC-SAXS three frames are averaged and analyzed for the three different peaks.