2-nitroimidazole nitrohydrolase (T2I, G14D, K73R)

NnhA protein

Organism: Mycobacterium sp. (strain JS330)

Monomeric molecular weight: 43.1 kDa

Oligomeric state: Hexamer

Total molecular weight: 258.5 kDa

UniProt: F4ZCI3 (1-379)

Sequence:

MHHHHHHMITVDKRPSSRGYDDWRLSDIPQYKDGISTYEFVRATHEADYRTHQAEPVAGRTFGFNGIGRLTEVALHMPTRYTLHDQSSQYKESPSFFQGLMGVPDRGPVDLAAFQRETEELATAFENNGIKVHWVDYPEEPANPYGPLMGHVFLSWGSIWRGGSVISRFGFLPGMVGVSEYLAKWAWNTLNIPPLVAITEGAMEPGACNMIADEVLVTCLSASYDQRGTDQLVAAISKTSGTEEFHNLQLRPAVEGFFNKATGACAhPdININAIDVGKLVVSPAALDWDARTWLYDNNFELIEADPDEQREFLAPCnVLLLEPGKVIAHADCHKTNQKIRDAGVEVIEVTGTEIRKAcGGIKcRVMQINREPGPTLADVRNRVWR

Soluble NnhA from Mycobacterium sp. JS330 (carrying the point mutations T2I, G14D K73R). The protein construct used for SAXS contains an additional non-native N-terminal polyhistidine tag (MHHHHHH).