Third double-stranded RNA-binding domain of human ADAR1 (interface mutant, residues 708-801, i.e. ADAR1-dsRBD3 interface mutant)

Mutant-ADAR1-dsRBD3 protein

Organism: Homo sapiens

Monomeric molecular weight: 12.5 kDa

Oligomeric state: Monomer

Total molecular weight: 12.5 kDa

UniProt: P55265 (708-801)

Sequence:

MGSSHHHHHHSSGLVPRGSHMMPNKVRKIGELVRYLNTNPVGGLLEYARSHGFAAEFKLAQQSGPPHEPKFVYQAKVGGRVFPAVSAHSKKQGKQEAADAALRVLIGENEKAER

construct contains 4 point mutations that render ADAR1-dsRBD3 monomeric, namely V747A+D748Q+W768V+C773S