N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF

SidF protein

Organism: Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)

Monomeric molecular weight: 55.7 kDa

Oligomeric state: Tetramer

Total molecular weight: 222.8 kDa

UniProt: Q4WF55 (1-462)

Sequence:

MGSSHHHHHHSSGENLYFQGHMATQSSTELPQINMTTAEPTSANKRTYPWVKLPHPYLTSYAIHVVSESTPRVLQLRLHDDQKGQALPEPLHSASLTYTDIAFDEAAQEIPDNDNSPWARSRRAPGTSFHWTGQEPPTLGQIWNVIHALFLTYPQHEIVRLDLNGSGKDIIREECLRTGLAVPFPSPRVPFGTENRPSETDTLILLRSAFWQGAGSPVGPRPIWAVDHGIHGLLRRSGSSYPPLAQNYEFSMKFPSERIYTRHPIRPQKPAPGSLVYSRYIPHLDQHFSLMVVDWQNEEHLQLFHKWQNDPRVAKGWNETGDLEHHRNYLRQLHEDKHVLCLFGRFDDFPFSYFEVYWAKEDHYGAHYDAGDYDRGRHSLVGESSVRGAYRVNAWWSSLIHYIFLDEPRTMCVVGEPKATNTTVLSYENAHGLTVQKYVDLGHKRSVHVYCSREKWFQLCPLFWDGRERPLESSDRMAWDAKL

The protein construct used for SAXS contains a non-native polyhistidine tag, with additional non-native amino acids at the N-terminus (MGSSHHHHHHSSGENLYFQGH).