The extracellular juncture domains in the intimin passenger adopt a constitutively extended conformation inducing restraints to its sphere of action.

Weikum J, Kulakova A, Tesei G, Yoshimoto S, Jægerum LV, Schütz M, Hori K, Skepö M, Harris P, Leo JC, Morth JP
Sci Rep 10(1):21249 (2020 Dec 4)
PMID: 33277518
doi: 10.1038/s41598-020-77706-7 		Submitted to SASBDB: 2020 Jun 10
Published in SASBDB:

SASDJ84 – Intimin D00-D0 domain

Intimin, D00-D0 domain (6xHis tagged) experimental SAS data
EOM/RANCH model
Sample: Intimin, D00-D0 domain (6xHis tagged) monomer, 23 kDa Escherichia coli O127:H6 … protein
Buffer: 10 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 12
RgGuinier 3.1 nm
Dmax 8.3 nm
VolumePorod 32 nm3

SASDJ94 – Intimin D0-D1 domain

Intimin (D0-D1 domain, 6xHis tagged) experimental SAS data
Intimin D0-D1 domain Rg histogram
Sample: Intimin (D0-D1 domain, 6xHis tagged) monomer, 23 kDa Escherichia coli O127:H6 … protein
Buffer: 10 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 12
RgGuinier 2.2 nm
Dmax 5.8 nm
VolumePorod 34 nm3